ABSTRACT
PURPOSE: To evaluate the effects of fat embolism in organs such as lung and liver. METHODS: Twenty rabbits underwent autologous intramuscular fat grafting in the right thigh after liposuction. The groups were determined according to the postoperative day that occurred in euthanasia: 60, 90 and 120 day. Then, lung and liver were excised and sent to the histopathology laboratory for analysis in search of late injury secondary to a prior event of fat embolism in the tissues. RESULTS: The results showed a change in the macroscopic sample with discoloration of the liver tissue heterogeneously. There were no changes consistent with embolic effect under the microscope. CONCLUSION: The option of performing a technique of fat grafting with a less traumatic surgical procedure can be considered protective against embolic events, with no impact to late embolic events on the tissues.
OBJETIVO: Avaliar a repercussão da embolia gordurosa em órgãos como pulmão e fígado. MÉTODOS: Vinte coelhos foram submetidos à enxertia autóloga intramuscular de gordura em coxa direita após lipoaspiração. Os grupos foram determinados conforme os dias pós-operatórios (DPO) em que ocorreu a eutanásia: 60 DPO, 90DPO, 120 DPO. Em seguida, o pulmão e o fígado foram ressecados e encaminhados ao laboratório de histopatologia para análise em busca de lesão tardia secundária a evento de embolia gordurosa prévia nos tecidos. RESULTADOS: Foi evidenciada uma alteração na amostra a analise macroscópica com alteração da coloração do tecido hepático de forma heterogênea. Não houve alterações compatíveis com repercussão de processo embólico à microscopia. CONCLUSÃO: A opção pela realização de uma técnica de lipoenxertia menos traumática e com pequeno tempo cirúrgico pode ser considerada protetora para eventos embólicos, não havendo repercussão embólica a tardiamente.
Subject(s)
Animals , Male , Rabbits , Adipose Tissue/transplantation , Embolism, Fat/etiology , Lipectomy/adverse effects , Liver/blood supply , Lung/blood supply , Disease Models, Animal , Embolism, Fat/pathology , Injections, Intramuscular , Lipectomy/methods , Postoperative PeriodABSTRACT
PURPOSE: To evaluate the donor site of adipocytes as well as histopathological alterations secondary to liposuction. METHODS: All animals underwent liposuction with a syringe on the right side of the back. While the left side of the back was used as control and did not undergo intervention. The 10 rabbits were divided into two groups A and B according the postoperative day which were submitted to euthanasia: 90 and 120 days. All adipose tissue from the donor site was analyzed and compared with the control macroscopic and light microscopy. Tissues were weighed and analyzed searching for histological changes and late inflammatory response to trauma such as fibrosis, fat necrosis and inflammation and macrophage infiltration. RESULTS: There was wide variation in adipose tissue volume between the experimental and the control on macroscopic analysis. The presence of histopathological changes was found in two samples at 90 days. CONCLUSIONS: There was a relationship between the presence of fibrosis with the weight and number of days after liposuction surgery in rabbits. The study show macroscopic difference between control and experiment sides in all rabbits.
OBJETIVO: Avaliar a zona doadora de adipócitos bem como as alterações histopatológicas secundárias a lipoaspiração. MÉTODOS: Todos os animais foram submetidos à lipoaspiração com seringa no lado direito do dorso. Enquanto o lado esquerdo do dorso foi usado como controle, não sofrendo intervenção. Os 10 coelhos foram divididos em dois grupos A e B conforme o dia pós-operatório (DPO) os quais foram submetidos à eutanásia: 90 DPO e 120 DPO. Todo tecido adiposo da zona doadora foi analisado e comparados com o controle macroscopicamente e à microscopia óptica. Os tecidos foram pesados e foram analisadas as variações histológicas em busca de uma resposta inflamatória tardia ao trauma como fibrose, inflamação, necrose gordurosa e infiltrado macrofágico. RESULTADOS: Foi observada grande variação de volume de tecido adiposo entre o experimento e o controle do dorso a macroscópica e a presença de alteração histopatológica em duas amostras aos 90 dias. CONCLUSÕES: Houve relação entre a presença de fibrose com o peso e numero de dias pós-operatório na lipoaspiração de coelhos. O estudo mostrou diferença entre os lados experimento e controle em todos os coelhos.
Subject(s)
Animals , Male , Rabbits , Adipose Tissue/pathology , Lipectomy/methods , Transplant Donor Site/pathology , Fibrosis , Inflammation/etiology , Inflammation/pathology , Lipectomy/adverse effects , Necrosis/etiology , Necrosis/pathology , Severity of Illness IndexABSTRACT
Post-kala-azar dermal leishmaniasis (PKDL) is rarely reported in South America. In spite of the fact that there are many reports about the association of visceral leishmaniasis and AIDS, PKDL is very uncommon in HIV-positive patients, and so far only four cases have been documented in the literature. We present another case with unusual clinicopathological aspects. The patient, a 28-year-old male, from Salvador, Bahia (an endemic area) presented with clinical manifestations of visceral leishmaniasis three years after the diagnosis of AIDS. During treatment for visceral leishmaniasis he developed disseminated miliary papules. Microscopically, the skin biopsy showed a "saw-tooth" pattern with a lichenoid mononuclear infiltrate simulating lichen planus. The histopathological diagnosis was achieved through the finding of amastigotes. The authors discuss the clinicopathological aspects of this case based on a review of the specific literature
Subject(s)
Male , Humans , Adult , Acquired Immunodeficiency Syndrome/complications , Antiprotozoal Agents , Leishmaniasis, Cutaneous , Leishmaniasis, VisceralABSTRACT
Post-kala-azar dermal leishmaniasis (PKDL) is rarely reported in South America. In spite of the fact that there are many reports about the association of visceral leishmaniasis and AIDS, PKDL is very uncommon in HIV-positive patients, and so far only four cases have been documented in the literature. We present another case with unusual clinicopathological aspects. The patient, a 28-year-old male, from Salvador, Bahia (an endemic area) presented with clinical manifestations of visceral leishmaniasis three years after the diagnosis of AIDS. During treatment for visceral leishmaniasis he developed disseminated miliary papules. Microscopically, the skin biopsy showed a "saw-tooth" pattern with a lichenoid mononuclear infiltrate simulating lichen planus. The histopathological diagnosis was achieved through the finding of amastigotes. The authors discuss the clinicopathological aspects of this case based on a review of the specific literature.